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KMID : 1134820200490101097
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Journal of the Korean Society of Food Science and Nutrition
2020 Volume.49 No. 10 p.1097 ~ p.1104
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Determination of Water-Soluble Carbohydrates in a Prime Core Set of Korean Rice Germplasms Using High-Performance Liquid Chromatography with Evaporative Light Scattering Detection
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Jeon Jin-Soo
Cho Byoung-Kwan
Kim Kyung-Hwan
Moon Jung-Kyung
Choung Myoung-Gun
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Abstract
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The profile of beneficial ingredients in major food crops is necessary to breed excellent cultivars and evaluate the nutritional value of foods. This study measured the levels of water-soluble carbohydrates in a prime core set of 401 Korean rice germplasms (345 typical brown rice, 42 red rice, and 14 black rice) using a high-performance liquid chromatography/evaporative light scattering detector (HPLC/ELSD). The water-soluble carbohydrate concentrations of the Korean rice germplasms were shown to be diverse in the range of the concentrations and compositions with concentrations ranging from 0.046 to 0.168% fructose, 0.056 to 0.284% glucose, 0.386 to 1.880% sucrose, 0.000 to 0.091% maltose, 0.000 to 0.254% raffinose, and 0.000 to 0.161% stachyose. In terms of the nutritional composition and functional characteristics, the Korean rice germplasms were confirmed to have genetic diversity, specificity, and superiority. A comparison of the total water-soluble carbohydrate concentrations by the color of rice revealed black rice to be arithmetically the highest at 1.46±0.23%, followed in order typical brown rice (1.43±0.31%) and red rice (1.25±0.26%). On the other hand, there was no significant difference between black rice and typical brown rice. Principal component analysis was performed using the water-soluble carbohydrate concentrations to select useful germplasms with a specific composition, concentration, and the outlier of the 95% confidence level and presented their line information. In addition, the characteristics and composition of water-soluble carbohydrate concentrations distribution of the Korean rice germplasms were reviewed comprehensively using correlation analysis, box-whisker plots, and a heatmap.
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KEYWORD
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water-soluble carbohydrates, rice, germplasms, HPLC/ELSD, PCA
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